Understanding the different ways in which epigenetic signals and transcription factor networks govern self-renewal, cell specification, differentiation, and reprogramming is a key step for advances in cell-based therapy and perspectives for medicine. Using mouse models, we aim to investigate how elimination and miss-expression of selected transcription factors affect epigenetic landscapes and downstream targets during embryonic development, particularly neuronal development. We will use Cre/loxP and global gene expression profiling (RNA sequencing), single cell sequencing, CUT&RUN chromatin profiling, FACS, confocal microscopy, immunohistochemistry, and in situ hybridization to evaluate molecular phenotypes and regulatory mechanisms.
Your qualifications and competences
- Completed Master’s or equivalent university degree in cell biology, genetics, biomedicine, molecular biology or related sciences.
- The candidate must be willing to work with murine models.
- Good command of written and spoken English
- Prior experience working with mice, microdissections, fluorescence microscopic methods, and knowledge of molecular methods and developmental biology and/or bioinformatics is a plus.
- Teamwork, motivated and enthusiastic, and excellent communication skills.
We offer to work in a creative, multidisciplinary international team. The laboratory of Molecular Pathogenetics is a part of the Institute of Biotechnology (IBT, www.ibt.cas.cz) located at the research centre BIOCEV, providing an inspiring multi-disciplinary research environment and access to state-of-the-art technologies and instruments. IBT/BIOCEV brings together > 500 scientists and students in > 50 research groups. Our laboratory collaborates with many European and US laboratories. We provide excellent training conditions for PhD students. S(he) will enroll in a PhD program at Faculty of Science at the Charles University in Prague. The PhD application must be submitted by December 15, 2023.
If you have any further questions, please do not hesitate to contact Dr. Gabriela Pavlinkova by e-mail: Tato e-mailová adresa je chráněna před spamboty. Pro její zobrazení musíte mít povolen Javascript..
Application materials: professional CV, indicating reference the Laboratory of Molecular Pathogenetics IBT. Please, send the required materials in electronic form to the e-mail address: Tato e-mailová adresa je chráněna před spamboty. Pro její zobrazení musíte mít povolen Javascript.. We look forward to receiving your application.
Publications to read:
- ISL1 is necessary for auditory neuron development and contributes towards tonotopic organization. PNAS 2022, Sept 8, 119 (37) e2207433119, https://doi.org/10.1073/pnas.2207433119.
- Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification. Nature Communications 2022, 13, 3878. https://doi.org/10.1038/s41467-022-31580-1.
- ISL1 controls pancreatic alpha cell fate and beta cell maturation. Cell & Bioscience 2023, 13, 53. https://doi.org/10.1186/s13578-023-01003-9.
More details on the processing of personal data are available on the website: https://www.ibt.cas.cz/cs/o-ustavu/oficialni-dokumenty/gdpr/.