Understanding the different ways in which epigenetic signals and transcription factor networks govern self-renewal, cell specification, differentiation, and reprogramming is a key step for advances in cell-based therapy and perspectives for medicine. Using mouse models, we aim to investigate how elimination and miss-expression of selected transcription factors affect epigenetic landscapes and downstream targets during embryonic development, particularly neuronal development. We will use Cre/loxP and global gene expression profiling (RNA sequencing), single cell sequencing, CUT&RUN chromatin profiling, FACS, confocal microscopy, immunohistochemistry, and in situ hybridization to evaluate molecular phenotypes and regulatory mechanisms.
Your qualifications and competences
- Completed Master’s or equivalent university degree in cell biology, genetics, biomedicine, molecular biology or related sciences.
- The candidate must be willing to work with murine models.
- Good command of written and spoken English
- Prior experience working with mice, microdissections, fluorescence microscopic methods, and knowledge of molecular methods and developmental biology and/or bioinformatics is a plus.
- Teamwork, motivated and enthusiastic, and excellent communication skills.
We offer to work in a creative, multidisciplinary international team. The laboratory of Molecular Pathogenetics is a part of the Institute of Biotechnology (IBT, www.ibt.cas.cz) located at the research centre BIOCEV, providing an inspiring multi-disciplinary research environment and access to state-of-the-art technologies and instruments. IBT/BIOCEV brings together > 500 scientists and students in > 50 research groups. Our laboratory collaborates with many European and US laboratories. We provide excellent training conditions for PhD students. S(he) will enroll in a PhD program at Faculty of Science at the Charles University in Prague. The PhD application must be submitted by December 15, 2023.
Publications to read:
- ISL1 is necessary for auditory neuron development and contributes towards tonotopic organization. PNAS 2022, Sept 8, 119 (37) e2207433119, https://doi.org/10.1073/pnas.2207433119.
- Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification. Nature Communications 2022, 13, 3878. https://doi.org/10.1038/s41467-022-31580-1.
- ISL1 controls pancreatic alpha cell fate and beta cell maturation. Cell & Bioscience 2023, 13, 53. https://doi.org/10.1186/s13578-023-01003-9.
More details on the processing of personal data are available on the website: https://www.ibt.cas.cz/cs/o-ustavu/oficialni-dokumenty/gdpr/.