Laboratory technician for the research group RNA-based Regulation of Gene Expression

A position for a laboratory technician/manager is available in the research group of Peter J Lukavsky, PhD “RNA-based Regulation of Gene Expression” which is part of Structural Biology program at CEITEC, Masaryk University (MU). We study disease-related biological processes by investigating protein structures and their interactions with other proteins, RNA or small molecule inhibitors using biomolecular NMR and related biophysical methods.

Job description:

  • Expression and purification of proteins
  • Transcription and purification of RNAs
  • Preparation of various media and buffers
  • PCR and cloning of RNA and protein constructs
  • Preparation of isotope-labelled nucleotides
  • Binding studies using ITC, fluorescence anisotropy, EMSA, and other biophysical techniques including data analysis
  • Performance of NMR experiments to study small molecule interactions with proteins and RNA
  • Implementing new techniques and projects
  • Help incoming co-workers into a smooth start in the lab
  • Ordering of chemicals and lab supplies

Requirements:

  • At least Master degree in molecular biology, biochemistry, biophysics, chemistry or other related fields of life-sciences
  • Good knowledge of English (oral and written)
  • Excellent interpersonal and communication skills
  • High motivation, flexibility and self-driven interest to learn new things
  • Reliability, team player, meticulousness
  • Knowledge of ""laboratory chemistry"" - preparation of solutions with defined concentrations, adjusting pH, preparation of media for bacterial cultures etc.
  • Computer literacy, MS word, Excel, Adobe Illustrator.
  • Excellent experience with molecular biology, protein and RNA purification, biochemistry and NMR spectroscopy is a big plus

We offer:

  • Interesting work in a top scientific team
  • Stable base and excellent instrumental equipment of CEITEC-MU
  • MU employment benefits
  • Salary based on qualification and experience; part time appointment possible

Please send CV and a short motivational letter including: a) description of previous experiences b) technical skills and c) at least two contact references should be sent by e-application available on https://www.muni.cz/en/about-us/careers/vacancies/66999.

In the case of any difficulties filling out the e-application, please contact Veronika Slezáková (Tato e-mailová adresa je chráněna před spamboty. Pro její zobrazení musíte mít povolen Javascript.).

Deadline of the applications is 31 August 2022.