Laboratory Technician for the Research Group RNA-based Regulation of Gene Expression

Laboratory Technician for the Research Group “RNA-based Regulation of Gene Expression”

Department Peter Lukavsky Research Group – Central European Institute of Technology   Research Group Leader: Peter Josef Lukavsky, Ph.D.   A position for a laboratory technician is available in the research group “RNA-based regulation of Gene Expression” which is part of Structural Biology program at CEITEC, Masaryk University (MU). We study disease-related biological processes by investigating protein structures and their interactions with other proteins, RNA or small molecule inhibitors using biomolecular NMR and related biophysical methods.   Job Description: - Expression and purification of proteins - Transcription and purification of RNAs - Preparation of various media and buffers - PCR and cloning of RNA and protein constructs - Preparation of isotope-labelled nucleotides - Binding studies using ITC, fluorescence anisotropy, EMSA, and other biophysical techniques including data analysis - Performance of NMR experiments to study small molecule interactions with proteins and RNA - Implementing new techniques and projects - Help incoming co-workers into a smooth start in the lab   Requirements: - At least Master degree in molecular biology, biochemistry, biophysics, chemistry or other related fields of life-sciences - Good knowledge of English (oral and written) - Excellent interpersonal and communication skills - High motivation, flexibility and self-driven interest to learn new things - Reliability, team player, meticulousness - Knowledge of "laboratory chemistry" - preparation of solutions with defined concentrations, adjusting pH, preparation of media for bacterial cultures etc. - Computer literacy, MS word, Excel. - Excellent experience with molecular biology, protein and RNA purifcation, biochemistry and NMR spectroscopy is a big plus   We offer: - Interesting work in a top scientific team - Stable base and excellent instrumental equipment of CEITEC MU - employment benefits - Salary based on qualification and experience; part time appointment possible   Brno is an attractive, lively place to live and work with many cultural and recreational amenities. Start date: Negotiable Please send CV and a short motivational letter including: a) description of previous experiences b) technical skills and c) at least two contact references to Peter Lukavsky (Tato e-mailová adresa je chráněna před spamboty. Pro její zobrazení musíte mít povolen Javascript.). Candidates considered for the second round of interview will be informed via email.